Our lab is interested in the systems biology and evolution of epigenetic switches (bistability) and clocks (oscillators) in gene regulatory networks. We use experiment and theory, biology and physics, systems and synthetic biology to study the cell cycle, metabolic rhythms, and circadian clocks. How do oscillators with different frequencies co-exist in the same cell? Are there mechanisms and regulatory principles that ensure functional harmony between oscillators?
His committee was Joshua Socolar (Physics), Gleb Finkelstein (Physics), Berndt Mueller (Physics), Xinnian Dong (Biology), and Nicolas Buchler (Physics & Biology). Here’s a picture of Sargis with Armenian brandy (after the thesis).
Selcan Aydin measured GAL1 gene expression at low galactose levels in single yeasts using timelapse luminescence microscope. Her data shows that the graded increase in GAL1 induction at the population level reflects a heterogeneous induction lag at the single cell level (click here to see movie). This work illustrates the power of single cell analysis using timelapse luminescence microscopy; see Mazo-Vargas et al, MBoC 2014. This was a quick collaboration with the Pascual-Ahuir and Proft labs.
Rienzo A, Poveda-Huertes D, Aydin S, Buchler NE, Pascual-Ahuir A, Proft M. Different mechanisms confer gradual control and memory at nutrient- and stress-regulated genes in yeast. Mol. Cell. Biol. 2015; 35: 3669-83.
Our lab was out in full force at the Ninth q-bio Conference in Blacksburg, Virginia.
- Heungwon Park, “A synthetic oscillator couples to the cell division cycle in budding yeast” [talk]
- Sargis Karapetyan “Redox rhythms reinforce the plant circadian clock” [poster]
- Tony Burnetti “The yeast metabolic cycle is coupled to cell division cycle Start across diverse strains” [poster].