Duke iGEM team wins gold at Giant Jamboree 2014

Screen Shot 2014-11-06 at 10.25.27Duke’s iGEM team traveled to compete at the Giant Jamboree in Boston on October 30th – November 3rd, 2014.  There were 2500 synthetic biology researchers (mostly undergraduates) from 245 universities across 32 countries.  Each team showcased their yearly achievements in synthetic biology at the iGEM Synthetic Biology competition.  Duke got gold!  Congratulations! Read more about their summer project here.

Delta Ghoshal, Mike Zhu, Matthew Faw, and Garima Tomar (NCSSM) represented Duke at the Giant Jamboree.  The team was mentored by Charlie Cooper (graduate student, Biochemistry) and generously funded by the Lord Alstadt Foundation. The faculty sponsors were Nicolas Buchler and Charles Gersbach.

Measuring fast gene dynamics in single cells using timelapse luminescence microscopy

Mazo-Vargas et al. was published in November 5th, 2014  issue of the Molecular Biology of the Cell.

Beetle luciferases and time-lapse luminescence microscopy were optimized to measure the dynamics of cell cycle genes in yeast with subminute time resolution. This method is faster and the cells are smaller than in previous work. It is shown that luciferase reporters are better than fluorescent proteins at tracking gene expression. Timelapse luminescence movie can be found at Duke Research Blog post here.