Measuring fast gene dynamics in single cells using timelapse luminescence microscopy

Mazo-Vargas et al. was published in November 5th, 2014  issue of the Molecular Biology of the Cell.

Beetle luciferases and time-lapse luminescence microscopy were optimized to measure the dynamics of cell cycle genes in yeast with subminute time resolution. This method is faster and the cells are smaller than in previous work. It is shown that luciferase reporters are better than fluorescent proteins at tracking gene expression. Timelapse luminescence movie can be found at Duke Research Blog post here.

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