Selcan Aydin measured GAL1 gene expression at low galactose levels in single yeasts using timelapse luminescence microscope. Her data shows that the graded increase in GAL1 induction at the population level reflects a heterogeneous induction lag at the single cell level (click here to see movie). This work illustrates the power of single cell analysis using timelapse luminescence microscopy; see Mazo-Vargas et al, MBoC 2014. This was a quick collaboration with the Pascual-Ahuir and Proft labs.
Rienzo A, Poveda-Huertes D, Aydin S, Buchler NE, Pascual-Ahuir A, Proft M. Different mechanisms confer gradual control and memory at nutrient- and stress-regulated genes in yeast. Mol. Cell. Biol. 2015; 35: 3669-83.